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ID: 16649.0, MPI für biophysikalische Chemie / NanoBiophotonik (Dr. Stefan Hell)
Axial superresolution with ultrahigh aperture lenses
Authors:Blanca, C. M.; Hell, S. W.
Language:English
Date of Publication (YYYY-MM-DD):2002-08-26
Title of Journal:Optics Express
Volume:10
Issue / Number:17
Start Page:893
End Page:898
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:We explore the current limits of the axial resolution of optical sectioning microscopy using a single lens, by combining the resolving power of novel 1.45 numerical aperture oil immersion lenses with superresolving binary aperture filters. We quantify the axial resolution brought about by the increase in semiaperture angle to alpha(max)=72.8degrees and demonstrate an absolute gain in axial resolution through binary pupil filters. Implemented in a confocalized two-photon excitation microscope, the combination of both effectively improves the axial resolution to 330 nm full-width-half-maximum, which is by 34% over that of a standard 1.4 NA system. (C) 2002 Optical Society of America
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
Identifiers:URL:http://www.opticsinfobase.org/oe/viewmedia.cfm?uri...
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