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ID: 16650.0, MPI für biophysikalische Chemie / Zelluläre Biochemie (Prof. Reinhard Lührmann)
Single-stranded antisense siRNAs guide target RNA cleavage in RNAi
Authors:Martinez, J.; Patkaniowska, A.; Urlaub, H.; Luehrmann, R.; Tuschl, T.
Language:English
Date of Publication (YYYY-MM-DD):2002-09-06
Title of Journal:Cell
Volume:110
Issue / Number:5
Start Page:563
End Page:574
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates siRNA- mediated target RNA degradation. By using affinity-tagged siRNAs, we demonstrate that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with eIF2C1 and/or eIF2C2 (human GERp95) Argonaute proteins. RISC is rapidly formed in HeLa cell cytoplasmic extract supplemented with 21 nt siRNA duplexes, but also by adding single-stranded antisense RNAs, which range in size between 19 and 29 nucleotides. Single-stranded antisense siRNAs are also effectively silencing genes in HeLa cells, especially when 5'- phosphorylated, and expand the repertoire of RNA reagents suitable for gene targeting.
Comment of the Author/Creator:Date: 2002, SEP 6
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Reinhard Lührmann / 100
MPI für biophysikalische Chemie/AG Thomas Tuschl
MPI für biophysikalische Chemie/AG Henning Urlaub
Identifiers:URL:http://www.sciencedirect.com/science?_ob=MImg&_ima...
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