Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Display Documents



  history
ID: 16914.0, MPI für biophysikalische Chemie / NanoBiophotonik (Dr. Stefan Hell)
Z-polarized confocal microscopy
Authors:Huse, N.; Schoenle, A.; Hell, S. W.
Language:English
Date of Publication (YYYY-MM-DD):2001-10
Title of Journal:Journal of Biomedical Optics
Journal Abbrev.:.
Volume:6
Issue / Number:4
Start Page:480
End Page:484
Review Status:not specified
Audience:Not Specified
Abstract / Description:In light microscopy the transverse nature of the electromagnetic field precludes a strongly focused longitudinal field component, thus confining polarization spectroscopy and imaging to two dimensions (xy). Here we describe a simple confocal microscopy arrangement that optimizes for signal from molecules with transition dipoles oriented parallel to the optic axis. In the proposed arrangement, we not only generate a predominant longitudinally (z) polarized focal field, but also engineer the detection scheme in such a way that in a bulk of randomly oriented molecules, the microscope's effective point- spread function is dominated by the contribution of those molecules that are oriented along the optic axis. Our arrangement not only implicitly allows for the determination of the orientation of transition dipoles of single molecules in three dimensions, but also highlights the contribution of z- oriented molecules in three-dimensional imaging. (C) 2001 Society of Photo-optical Instrumentation Engineers
Free Keywords:single molecule spectroscopy; longitudinal field; microscopy; confocal; transition dipole; z polarization
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
Identifiers:URL:http://spiedigitallibrary.org/getpdf/servlet/GetPD...
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.