Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Display Documents



  history
ID: 17092.0, MPI für biophysikalische Chemie / Membranbiophysik (Prof. Erwin Neher)
Separation of presynaptic and postsynaptic contributions to depression by covariance analysis of successive EPSCs at the calyx of Held synapse
Authors:Scheuss, V.; Schneggenburger, R.; Neher, E.
Language:English
Date of Publication (YYYY-MM-DD):2002-02-01
Title of Journal:Journal of Neuroscience
Volume:22
Issue / Number:3
Start Page:728
End Page:739
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Synaptic short-term plasticity is considered to result from multiple cellular mechanisms, which may include presynaptic and postsynaptic contributions. We have recently developed a non- stationary EPSC fluctuation analysis (Scheuss and Neher, 2001) to estimate synaptic parameters and their transient changes during short-term synaptic plasticity. Extending the classical variance-mean approach, a short train of stimuli is applied repetitively, and the resulting EPSCs are analyzed for means, variances, and covariances. This provides estimates of the quantal size and quantal content for each EPSC in the train, and furthermore, an estimate of the number of release sites. The latter is less sensitive to heterogeneity in the release probability than that of the variance-mean approach. Here, we applied this analysis to the calyx of Held synapse in brainstem slices of young rats (postnatal day 8-10). We found significant negative covariance in the amplitude of successive EPSCs in a train. The analysis showed that the 10-fold depression in the EPSC amplitude during 100 Hz trains at elevated extracellular Ca2+ concentration resulted from a 2.5-fold reduction in quantal size caused by postsynaptic AMPA receptor desensitization and saturation, and a fourfold reduction in quantal content, which was partially relieved by application of cyclothiazide. The number of release sites estimated by covariance analysis was approximate to2000 and significantly larger than estimates from variance-mean parabolas.
Free Keywords:synaptic transmission; short-term plasticity; quantal analysis; covariance analysis; release site; release probability; quantal size
Comment of the Author/Creator:Date: 2002, FEB 1
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Erwin Neher / 140
Identifiers:URL:http://www.jneurosci.org/cgi/reprint/22/3/728
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.