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ID: 17125.0, MPI für biophysikalische Chemie / NMR-basierte Strukturbiologie (Prof. Christian Griesinger)
Mass spectrometric Sequencing of individual peptides from combinatorial libraries via specific generation of chain- terminated sequences
Authors:Hoffmann, C.; Blechschmidt, D.; Kruger, R.; Karas, M.; Griesinger, C.
Language:English
Date of Publication (YYYY-MM-DD):2002-01
Title of Journal:Journal of Combinatorial Chemistry
Volume:4
Issue / Number:1
Start Page:79
End Page:86
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Combinatorial peptide libraries are a versatile tool for drug discovery. On-bead assays identify reactive peptides by enzyme- catalyzed staining and, usually, sequencing by Edman degradation. Unfortunately, the latter method is expensive and time-consuming and requires free N termini of the peptides. A method of rapid and unambiguous peptide sequencing by utilizing synthesis-implemented generation of termination sequences with subsequent matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis is introduced here. The required capped sequences are determined and optimized for a specific peptide library by a computer algorithm implemented in the program Biblio. A total of 99.7% of the sequences of a heptapeptide library sample could be decoded utilizing a single bead for each spectrum. To synthesize these libraries, an optimized capping approach has been introduced.
Comment of the Author/Creator:Date: 2002, JAN-FEB
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Christian Griesinger / 030
External Affiliations:Univ Frankfurt, Inst Organ Chem, Marie Curie Str 11, D-60439; Frankfurt, Germany
Identifiers:URL:http://pubs.acs.org/doi/pdfplus/10.1021/cc010057x
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