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ID: 176064.0, MPI für Evolutionsbiologie / Evolutionary ecology
Evaluation of an innate immune reaction to parasites in earthworms
Authors:Field, Stuart G.; Kurtz, Joachim; Cooper, Edwin L.; Michiels, Nico K.
Language:English
Date of Publication (YYYY-MM-DD):2004-05
Title of Journal:Journal of Invertebrate Pathology
Volume:86
Issue / Number:1-2
Start Page:45
End Page:49
Review Status:not specified
Audience:Not Specified
Abstract / Description:Encapsulation is an essential process of the invertebrate immune system and includes the prophenoloxidase (proPO) cascade. We present an assay for evaluating this immune response, now newly adapted to earthworms. Coelomic fluid is withdrawn and coelomocytes are stained with L-Dopa. We studied assay repeatability and the correlation between number of PO-active cells and infection level of the parasitic protozoan Monocystis sp. in the earthworm Lumbricus terrestris. Our study showed high assay repeatability although the expected negative relationship between PO-active coelomocytes and parasite load was not observed; yet a suggestion toward a positive relationship was detected. This finding is contrary to previous assumptions that presume coelomocyte concentrations to be the independent variable determining parasite load.
Free Keywords:immunocompetence; phenoloxidase; innate immunity; Lumbricus terrestris; coelomocytes; immunoassay; L-3,4-dihydroxyphenylalanine; encapsulation; Monocystis sp.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Limnologie/Abt. Evolutionsökologie
External Affiliations:Univ Münster, Inst Anim Ecol & Evolut, Münster, D-48149 Germany; Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurobiol, Lab Comparat Neuroimmunol, Los Angeles, CA 90095 USA
Identifiers:ISSN:0022-2011 [ID-No:1]
DOI:10.1016/j.jip.2004.04.001 [ID-No:2]
LOCALID:2298/S 38173 [Listen-Nummer/S-Nummer]
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