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ID: 194849.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
The properties of the single chicken MHC classical class II alpha chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules
Authors:Salomonsen, Jan; Marston, Denise; Avila, David; Bumstead, Nat; Johansson, Brit; Juul-Madsen, Helle; Olesen, Gerda D.; Riegert, Patricia; Skjodt, Karsten; Vainio, Olli; Wiles, Michael V.; Kaufman, Jim
Language:English
Date of Publication (YYYY-MM-DD):2003-12
Title of Journal:Immunogenetics
Journal Abbrev.:Immunogenetics
Volume:55
Issue / Number:9
Start Page:605
End Page:614
Copyright:Springer-Verlag Heidelberg
Review Status:not specified
Audience:Experts Only
Abstract / Description:In mammals, there are MHC class II molecules with distinctive sequence features, such as the classical isotypes DR, DQ and DP. These particular isotypes have not been reported in non-mammalian vertebrates. We have isolated the class II (B-L) chain from outbred chickens as the basis for the cloning and sequencing of the cDNA. We found only one class II chain transcript, which bears the major features of a classical class II sequence, including the critical peptide-binding residues. The chicken sequence is more similar to human DR than to the DQ, DP, DO or DM isotypes, most significantly in the peptide-binding 1 domain. The cDNA and genomic DNA sequences from chickens of diverse origins show few alleles, which differ in only four nucleotides and one amino acid. In contrast, significant restriction fragment length polymorphism is detected by Southern blot analysis of genomic DNA, suggesting considerable diversity around the gene. Analysis of a large back-cross family indicates that the class II chain locus (B-LA) is located roughly 5.6 cM from the MHC locus, which encodes the classical class II chains. Thus the chicken class II chain gene is like the mammalian DR and E isotypes in three properties: the presence of the critical peptide-binding residues, the low level of polymorphism and sequence diversity, and the recombinational separation from the class II chain genes. These results indicate that the sequence features of this lineage are both functionally important and at least 300 million years old.
Free Keywords:major histocompatibility complex, B locus, class II, DR isotype, chicken
Comment of the Author/Creator:Published online: 8 November 2003
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Inst Anim Hlth, Compton RG20 7NN, Berks, England.; Royal Vet & Agr Univ, Dept Virol & Immunol, Stigbojlen 7, DK-1870 Frederiksberg, Denmark.; Basel Inst Immunol, CH-4005 Basel, Switzerland.; Roskilde Univ Ctr, Dept Chem & Life Sci, DK-4000 Roskilde, Denmark.; Odense Univ, Dept Immunol & Med Microbiol, DK-5000 Odense, Denmark.; Turku Univ, Dept Med Microbiol, FIN-20520 Turku, Finland.; Max Planck Inst Mol Genet, D-14195 Berlin, Germany.
Identifiers:ISI:000187288900003 [ID No:1]
ISSN:0093-7711 [ID No:2]
DOI:10.1007/s00251-003-0620-7 [ID No:3]
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