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ID: 206547.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Ensemble and single particle photophysical properties (Two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
Authors:Grecco, H. E.; Lidke, K. A.; Heintzmann, R.; Lidke, D. S.; Spagnuolo, C.; Martinez, O. E.; Jares-Erijman, E. A.; Jovin, T. M.
Language:English
Date of Publication (YYYY-MM-DD):2004-12-22
Title of Journal:Miscroscopy Research and Technique
Volume:65
Start Page:169
End Page:179
Sequence Number of Article:DOI 10.1002/jemt.20129
Copyright:c* 2004 WILEY-LISS, INC.
Review Status:not specified
Audience:Not Specified
Abstract / Description:In this work we characterized streptavidin conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (i) two-photon excitation; (ii) fluorescence lifetimes; (iii) ensemble and single QD emission anisotropy; (iv) QDs as donors for Förster resonance energy transfer (FRET); and (v) spectral conversion of QDs exposed to high intensity illumination. We also demonstrate the utility of QDs for (i) imaging the binding and uptake of biotinylated transferrin on living cells, and (ii) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs).
Free Keywords:nanoparticles; fluorescence microscopy; growth factor receptors
Last Change of the Resource (YYYY-MM-DD):2005-01-03
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Laboratorio de Electrónica Cuántica, Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón I, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina; Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Identifiers:URL:http://www3.interscience.wiley.com/cgi-bin/fulltex...
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