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ID: 206756.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
One- and two-photon photoactivation of a paGFP-fusion protein, a phototoxicity study in live Drosophila embryos
Authors:Post, J. N.; Lidke, K. A.; Rieger, B.; Arndt-Jovin, D. J.
Language:English
Date of Publication (YYYY-MM-DD):2005-01-17
Title of Journal:FEBS Letters
Volume:579
Start Page:325
End Page:330
Sequence Number of Article:doi:10.1016/j.febslet.2004.11.092
Review Status:not specified
Audience:Not Specified
Abstract / Description:We constructed a photoactivatable Drosophila histone 2 A variant green fluorescent fusion protein (H2AvDpaGFP) for tracking chromatin loci in living Drosophila embryos. Activation of paGFP was achieved by irradiation from a single-photon diode laser at 408 nm, but activated nuclei failed to divide. Photoconversion could also be achieved by two-photon fs pulses in the range of 780–840 nm. Viability in whole-mount embryos could only be maintained at 820 nm, at which we could activate, simultaneously track and quantitate the mobility of multiple fluorescent loci. This report constitutes the first demonstration of two-photon activation of paGFP and the use of a paGFP-fusion protein in investigations of whole organisms.
Free Keywords:Green fluorescent protein; Histone; Chromatin dynamics; Nuclear architecture; Two-photon microscopy; Photoactivation
Last Change of the Resource (YYYY-MM-DD):2005-01-11
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060/AG Donna Arndt-Jovin
Identifiers:URL:http://www.sciencedirect.com/science?_ob=MImg&_ima...
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