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ID: 226580.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Interferometric measurement of fluorescence excitation spectra
Authors:Hirschberg, J. G.; Vereb, G.; Meyer, C. K.; Kirsch, A. K.; Kohen, E.; Jovin, T. M.
Date of Publication (YYYY-MM-DD):1998
Title of Journal:Applied Optics
Start Page:1953
End Page:1957
Copyright:Jahrbuch 1999, Copyright MPG 1999
Review Status:not specified
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:A Michelson interferometer has been adapted as an excitation source for fluorescence spectroscopy. A moving fringe pattern was generated by linear displacement of the movable mirror of the Michelson interferometer coupled to a xenon-arc lamp. This spectrally modulated source was monitored by a reference photomultiplier and used for exciting a Rhodamine B solution. The fluorescence emission at >645 nm was detected by a second photomultiplier. The two interferograms were acquired by a dual-channel digital oscilloscope, and their discrete Fourier transforms and corresponding power spectra were generated in a computer. The power spectrum of the emission signal represented the excitation spectrum, as was confirmed by comparison with the absorption spectrum of Rhodamine B. This optical arrangement is well suited for acquiring fluorescence excitation spectra in the optical microscopy of biological specimens.
Last Change of the Resource (YYYY-MM-DD):2005-07-06
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Department of Physics, University of Miami, Coral Gables, Florida 33124; Department of Biology, University of Miami, Coral Gables, Florida 33124; Humboldt Universität zu Berlin, Medizinische Fakultät Charité, Institut für Medizinische Physik und Biophysik, Ziegelstrasse 5–9 10098 Berlin, Germany; Department of Biophysics and Cell Biology, University Medical School of Debrecen, H4012 Debrecen, Hungary.
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