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ID: 226673.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy.
Authors:Jakobs, S.; Subramaniam, V.; Schoenle, A.; Jovin, T. M.; Hell, S. W.
Date of Publication (YYYY-MM-DD):2000
Title of Journal:FEBS Letters
Start Page:131
End Page:135
Copyright:Jahrbuch 2001, Copyright MPG 2001
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:The green fluorescent protein (GFP) has become an invaluable marker for monitoring protein localization and gene expression in vivo. Recently a new red fluorescent protein (drFP583 or DsRed), isolated from tropical corals, has been described [Matz, M.V. et al, (1999) Nature Biotech, 17, 969-973]. With emission maxima at 509 and 583 nm respectively, EGFP and DsRed are suited for almost crossover free dual color labeling upon simultaneous excitation, We imaged mixed populations of Escherichia coli expressing either EGFP or DsRed by one-photon confocal and by two-photon microscopy, Both excitation modes proved to be suitable for imaging cells expressing either of the fluorescent proteins. DsRed had an extended maturation time and E, coli expressing this fluorescent protein were significantly smaller than those expressing EGFP, In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy.
Free Keywords:2-photon excitation; Imaging microscopy; Protein; Gfp; Resolution; Mutants; Cells; green fluorescent protein; red fluorescent protein; confocal; Two-photon; fluorescence lifetime microscopy; Escherichia coli; E. coli
Last Change of the Resource (YYYY-MM-DD):2005-08-17
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
MPI für biophysikalische Chemie/AG Stefan Jakobs
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