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ID: 228246.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET)
Authors:Jares-Erijman, E. A.; Giordano, L.; Spagnuolo, C.; Lidke, K. A.; Jovin, T. M.
Date of Publication (YYYY-MM-DD):2005-06
Title of Journal:Molecular Crystals and Liquid Crystals
Volume:430
Start Page:257
End Page:265
Sequence Number of Article:DOI: 10.1080/15421400590946479
Review Status:not specified
Audience:Not Specified
Abstract / Description:The reversible modulation of the emission of CdSe=ZnS semiconductor nanocrystals (quantum dots) was achieved by binding photochromic diheteroarylethenesand switchable acceptors for fluorescence resonance energy transfer. A biotinylated diheteroarylethene derivative was bound to quantum dots bearing conjugated streptavidin, leading to an intensity decrease as a consequence of energy transfer to the closed form of the acceptor. Interconversion between the open and closed forms by irradiation with 365 and 546nm light enabled deactivation and activation, respectively, of the FRET process with a corresponding modulation of quantum dot emission, observed both in solution and by sequential wide-field imaging.
Free Keywords:diheteroarylethene; photochromism; quantum dot; FRET; pcFRET
Last Change of the Resource (YYYY-MM-DD):2005-06-21
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, PROPLAME-CONICET, Ciudad Universitaria-Pabellon, Buenos Aires, Argentina
Identifiers:URL:http://www.informaworld.com/smpp/ftinterface~conte...
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