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ID: 233162.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Reaching out for signals: Filopodia sense EGF and respond by directed retrograde transport of activated receptors
Authors:Lidke, D. S.; Lidke, K. A.; Rieger, B.; Jovin, T. M.; Arndt-Jovin, D. J.
Date of Publication (YYYY-MM-DD):2005-08-15
Title of Journal:Journal of Cell Biology
Issue / Number:4
Start Page:619
End Page:626
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:ErbB1 receptors situated on cellular filopodia undergo systematic retrograde transport after binding of the epidermal growth factor (EGF) and activation of the receptor tyrosine kinase. Specific inhibitors of the erbB1 receptor tyrosine kinase as well as cytochalasin D, a disruptor of the actin cytoskeleton, abolish transport but not free diffusion of the receptor–ligand complex. Diffusion constants and transport rates were determined with single molecule sensitivity by tracking receptors labeled with EGF conjugated to fluorescent quantum dots. Retrograde transport precedes receptor endocytosis, which occurs at the base of the filopodia. Initiation of transport requires the interaction and concerted activation of at least two liganded receptors and proceeds at a constant rate mediated by association with actin. These findings suggest a mechanism by which filopodia detect the presence and concentration of effector molecules far from the cell body and mediate cellular responses via directed transport of activated receptors.
Free Keywords:epidermal growth factor; EGF; epidermal growth factor receptor; EGFR; erbB1; retrograde transport; quantum dots
Last Change of the Resource (YYYY-MM-DD):2005-08-16
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060/AG Donna Arndt-Jovin
External Affiliations:FEI Electron Optics, Bldg. AAEp, 5600 KA Eindhoven, The Netherlands
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