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ID: 233177.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
The role of photon statistics in fluorescence anisotropy imaging
Authors:Lidke, K. A.; Rieger, B.; Jovin, T. M.; Heintzmann, R.
Language:English
Date of Publication (YYYY-MM-DD):2005-09
Title of Journal:IEEE Transactions on Image Processing
Volume:14
Issue / Number:9
Start Page:1237
End Page:1245
Review Status:Peer-review
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:Anisotropy imaging can be used to image resonance energy transfer between pairs of identical fluorophores and, thus, constitutes a powerful tool for monitoring protein homo-association in living single cells. The requirement for only a single fluorophore significantly simplifies biological preparation and interpretation. We use quantitative methods for the acquisition and image processing of anisotropy data that return the expected error of the anisotropy per pixel based on photon statistics. The analysis methods include calibration procedures and allow for a balance in spatial, anisotropy, and temporal resolution. They are featured here with anisotropy images of fluorescent calibration beads and enhanced green fluorescent protein complexes in live cells.
Free Keywords:Confocal microscopy; energy migration fluorescence resonance energy transfer; emFRET; enhanced green fluorescent protein (eGFP); scale selective filtering
Last Change of the Resource (YYYY-MM-DD):2005-09-13
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, U.K.
Identifiers:URL:http://ieeexplore.ieee.org/stamp/stamp.jsp?arnumbe...
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