Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Display Documents



  history
ID: 245293.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Tangential flow microfiltration and ultrafiltration for human influenza A virus concentration and purification
Authors:Wickramasinghe, S. R.; Kalbfuss, B.; Zimmermann, A.; Thom, V.; Reichl, U.
Language:English
Date of Publication (YYYY-MM-DD):2005
Title of Journal:Biotechnology and Bioengineering
Volume:92
Issue / Number:2
Start Page:199
End Page:208
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Large scale purification of viruses and viral vectors for gene therapy applications and viral vaccines is a major separation challenge. Here tangential flow microfiltration and ultrafiltration using flat sheet membranes has been investigated for concentration of human influenza A virus. Ultrafiltration membranes with molecular weight cutoffs of 100 and 300 kDa as well as 0.1, 0.2 and 0.45 mu m microfiltration membranes have been tested. The results indicate that use of 300 kDa membranes not only concentrate the virus particles but also lead to a significant removal of host cell proteins and DNA in the permeate. Tangential flow filtration may be used to fractionate virus particles. Human influenza A virus particles are spherical with an average size of 100 nm. Use of a 0.1 mu m membrane leads to passage of virus particles less than 100 nm into the permeate and an increase of larger particles in the retentate. These results suggest that control of the transmembrane pressure, membrane pore size and pore size distribution could enable isolation of intact virus particles from damaged virions. Isolation of the virus particles of interest from viral fragments and other particulate matter could result in simplification of subsequent purification steps. Larger pore size membranes such as 0.45 mu m that allow the passage of all virus particles may be used to remove host cell fragments. In addition virus particles attached to these fragments will be removed. Careful selection of membrane morphology and operating conditions will be essential in order to maximize the benefit of tangential flow filtration steps in the purification of viral products from cell cultures. (c) 2005 Wiley Periodicals, Inc.
External Publication Status:published
Document Type:Article
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
External Affiliations:Department of Chemical Engineering, Colorado State University, Fort Collins, Colorado 80523-1370; telephone: +1 970 491 5276; fax: +1 970 491 7369

Sartorius AG, Göttingen, Germany
Identifiers:URL:http://dx.doi.org/10.1002/bit.20599
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.