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ID: 24714.0, MPI für molekulare Genetik / Ribosome Research Groups
How does tmRNA move through the ribosome?
Authors:Ivanov, Pavel V.; Zvereva, Maria I.; Shpanchenko, Olga V.; Dontsova, Olga A.; Bogdanov, Alexey A.; Aglyamova, Galina V.; Lim, Valery I.; Teraoka, Yoshika; Nierhaus, Knud H.
Language:English
Date of Publication (YYYY-MM-DD):2002-01-25
Title of Journal:FEBS Letters
Volume:514
Issue / Number:1
Start Page:55
End Page:59
Review Status:not specified
Audience:Experts Only
Abstract / Description:To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon–anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.
Free Keywords:tmRNA; Ribosome; Translation; Cross-linking; Site-directed mutagenesis
External Publication Status:published
Document Type:Article
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Chemistry, M.V. Lomonosov Moscow State University, 119899 Moscow, Russia
Engelhardt Institute of Molecular Biology, Russian Academy of Science, Vavilov str. 32, 119991 Moscow, Russia
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