MPI für molekulare Genetik / Department of Vertebrate Genomics |
|Functional genomics using high-throughput RNA interference|
|Authors:||Vanhecke, Dominique; Janitz, Michal|
|Date of Publication (YYYY-MM-DD):||2005-02-10|
|Title of Journal:||Drug Discovery Today|
|Journal Abbrev.:||Drug Discov Today|
|Issue / Number:||3|
|Copyright:||© 2005 Elsevier Ltd|
|Review Status:||not specified|
|Abstract / Description:||RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
The review provides a comprehensive overview on current RNA interference-based high throughput genomic technologies. The cell array- and microwell plate-based loss-of-function cell assays are presented.
|Free Keywords:||Gene expression; Cell arrays; Transfection; Microarray|
|Comment of the Author/Creator:||Email: firstname.lastname@example.org|
|External Publication Status:||published|
|Communicated by:||Hans Lehrach|
|Affiliations:||MPI für molekulare Genetik|