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ID: 276165.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Functional genomics using high-throughput RNA interference
Authors:Vanhecke, Dominique; Janitz, Michal
Language:English
Date of Publication (YYYY-MM-DD):2005-02-10
Title of Journal:Drug Discovery Today
Journal Abbrev.:Drug Discov Today
Volume:10
Issue / Number:3
Start Page:205
End Page:212
Copyright:© 2005 Elsevier Ltd
Review Status:not specified
Audience:Experts Only
Abstract / Description:RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
The review provides a comprehensive overview on current RNA interference-based high throughput genomic technologies. The cell array- and microwell plate-based loss-of-function cell assays are presented.
Free Keywords:Gene expression; Cell arrays; Transfection; Microarray
Comment of the Author/Creator:Email: janitz@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
Identifiers:ISSN:1359-6446
DOI:10.1016/S1359-6446(04)03352-5
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