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ID: 28991.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Monitoring the Switch from Housekeeping to Pathogen Defense Metabolism in Arabidopsis thaliana Using cDNA Arrays
Authors:Scheideler, Marcel; Schlaich, Nikolaus L.; Fellenberg, Kurt; Beissbarth, Tim; Hauser, Nicole C.; Vingron, Martin; Slusarenko, Alan J.; Hoheisel, Jörg D.
Language:English
Date of Publication (YYYY-MM-DD):2002-03-22
Title of Journal:Journal of Biological Chemistry
Volume:277
Issue / Number:12
Start Page:10555
End Page:10561
Review Status:not specified
Audience:Experts Only
Abstract / Description:Plants respond to pathogen attack by deploying several defense reactions. Some rely on the activation of preformed components, whereas others depend on changes in transcriptional activity. Using cDNA arrays comprising 13,000 unique expressed sequence tags, changes in the transcriptome of Arabidopsis thaliana were monitored after attempted infection with the bacterial plant pathogen Pseudomonas syringae pv. tomato carrying the avirulence gene avrRpt2. Sampling at four time points during the first 24 h after infiltration revealed significant changes in the steady state transcript levels of ~650 genes within 10 min and a massive shift in gene expression patterns by 7 h involving ~2,000 genes representing many cellular processes. This shift from housekeeping to defense metabolism results from changes in regulatory and signaling circuits and from an increased demand for energy and biosynthetic capacity in plants fighting off a pathogenic attack. Concentrating our detailed analysis on the genes encoding enzymes in glycolysis, the Krebs cycle, the pentose phosphate pathway, the biosynthesis of aromatic amino acids, phenylpropanoids, and ethylene, we observed interesting differential regulation patterns. Furthermore, our data showed potentially important changes in areas of metabolism, such as the glyoxylate metabolism, hitherto not suspected to be components of plant defense.
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Functional Genome Analysis and Theoretical Bioinformatics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany
Institut für Pflanzenphysiologie (Bio III), Worringerweg 1, RWTH Aachen, D-52074 Aachen, Germany
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