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ID: 298309.0, MPI für Dynamik komplexer technischer Systeme / Physical and Chemical Foundations of Process Engineering
Chromatographic enantioseparation of amino acids using a new chiral stationary phase based on a macrocyclic glycopeptide antibiotic
Authors:Petrusevska, K.; Kuznetsov, M. A.; Gedicke, K.; Meshko, V.; Staroverov, S. M.; Seidel-Morgenstern, A.
Date of Publication (YYYY-MM-DD):2006
Title of Journal:Journal of Separation Science
Issue / Number:10 - Special Issue : Chromatographic Chiral Separations
Start Page:1447
End Page:1457
Review Status:not specified
Audience:Experts Only
Abstract / Description:The separation of the enantiomers of several α-amino acids was studied on a new chiral stationary phase (CSP) which is based on the macrocyclic glycopeptide antibiotic eremomycin attached to silica particles. Retention and separation factors were determined under analytical conditions at ambient temperature for different mobile phase compositions. In order to evaluate the potential with respect to preparative separations the adsorption isotherms of D- and L-methionine were determined for one mobile phase composition applying the elution by characteristic point method. The isotherms were validated by comparing experimentally determined elution profiles with predictions based on the equilibrium dispersive model. Finally, the performance of the eremomycin CSP was compared with a commercially available CSP based on the macrocyclic antibiotic teicoplanin. After determining the isotherms of D- and L-methionine also for the teicoplanin phase, the equilibrium dispersive model was used for both CSP to identify optimal operating conditions. For the separation and conditions considered the new eremomycin CSP revealed a better performance compared to the teicoplanin CSP.

Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim [accessed February 8th 2013]
Free Keywords:Amino acids; Chromatographic enantioseparation; Eremomycin; Macrocyclic glycopeptide antibiotics
External Publication Status:published
Document Type:Article
Communicated by:Andreas Seidel-Morgenstern
Affiliations:MPI für Dynamik komplexer technischer Systeme/Physical and Chemical Foundations of Process Engineering
External Affiliations:Faculty of Technology and Metallurgy, Ss. Cyril and Methodius University, Republic of Macedonia
Chemistry Department, Lomonosov State University, Russian Federation
Institut für Verfahrenstechnik, Otto-von-Guericke-Universität Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany
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