Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Display Documents



  history
ID: 429148.0, MPI für biophysikalische Chemie / Physikalische Biochemie (Prof. Marina Rodnina)
Distinct functions of elongation factor G in ribosome recycling and translocation.
Authors:Savelsbergh, A.; Rodnina, M. V.; Wintermeyer, W.
Language:English
Date of Publication (YYYY-MM-DD):2009-05
Title of Journal:RNA
Volume:15
Issue / Number:5
Start Page:772
End Page:780
Review Status:not specified
Audience:Not Specified
Abstract / Description:Elongation factor G (EF-G) promotes the translocation step in bacterial protein synthesis and, together with ribosome recycling factor (RRF), the disassembly of the post-termination ribosome. Unlike translocation, ribosome disassembly strictly requires GTP hydrolysis by EF-G. Here we report that ribosome disassembly is strongly inhibited by vanadate, an analog of inorganic phosphate (Pi), indicating that Pi release is required for ribosome disassembly. In contrast, the function of EF-G in single-round translocation is not affected by vanadate, while the turnover reaction is strongly inhibited. We also show that the antibiotic fusidic acid blocks ribosome disassembly by EF-G/RRF at a 1000-fold lower concentration than required for the inhibition of EF-G turnover in vitro and close to the effective inhibitory concentration in vivo, suggesting that the antimicrobial activity of fusidic acid is primarily due to the direct inhibition of ribosome recycling. Our results indicate that conformational coupling between EF-G and the ribosome is principally different in translocation and ribosome disassembly. Pi release is not required for the mechanochemical function of EF-G in translocation, whereas the interactions between RRF and EF-G introduce tight coupling between the conformational change of EF-G induced by Pi release and ribosome disassembly.
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für biophysikalische Chemie/Abt. Marina Rodnina
MPI für biophysikalische Chemie/AG Wolfgang Wintermeyer
Identifiers:URL:http://rnajournal.cshlp.org/content/15/5/772.full....
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.