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ID: 457851.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
An Iterative Strategy for Precursor Ion Selection for LC-MS/MS Based Shotgun Proteomics.
Authors:Zerck, Alexandra; Nordhoff, Eckhard; Resemann, Anja; Mirgorodskaya, Ekaterina; Suckau, Detlef; Reinert, Knut; Lehrach, Hans; Gobom, Johan
Language:English
Date of Publication (YYYY-MM-DD):2009-04-30
Title of Journal:Journal of Proteome Research
Journal Abbrev.:J. Proteome Res.
Volume:8
Issue / Number:7
Start Page:3239
End Page:3251
Copyright:2009 American Chemical Society
Review Status:not specified
Audience:Experts Only
Abstract / Description:Currently, the precursor ion selection strategies in LC-MS mainly choose the most prominent peptide signals for MS/MS analysis. Consequently, high-abundance proteins are identified by MS/MS of many peptides, whereas proteins of lower abundance might elude identification. We present a novel, iterative and result-driven approach for precursor ion selection that significantly increases the efficiency of an MS/MS analysis by decreasing data redundancy and analysis time. By simulating different strategies for precursor ion selection on an existing data set, we compare our method to existing result-driven strategies and evaluate its performance with regard to mass accuracy, database size, and sample complexity.
Comment of the Author/Creator:To whom correspondence should be addressed.
E-mail: zerck@molgen.mpg.de.
Max Planck Institute for Molecular Genetics.
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
Identifiers:URL:http://pubs.acs.org/doi/pdf/10.1021/pr800835x
DOI:10.1021/pr800835x
ISSN:1535-3893
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