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ID: 476830.0, MPI für Dynamik und Selbstorganisation / Hydrodynamik und Strukturbildung
Live cell flattening - traditional and novel approaches
Authors:Westendorf, Christian; Bae, Albert; Erlenkamper, Christoph; Galland, Edouard; Franck, Carl; Bodenschatz, Eberhard; Beta, Carsten
Language:English
Date of Publication (YYYY-MM-DD):2010-04-19
Title of Journal:PMC Biophysics
Journal Abbrev.:PMC Biophys.
Volume:3
Start Page:9-1
End Page:9-15
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.
External Publication Status:published
Document Type:Article
Communicated by:Folkert Müller-Hoissen
Affiliations:MPI für Dynamik und Selbstorganisation/Hydrodynamik und Strukturbildung
External Affiliations:Laboratory of Atomic and Solid-State Physics, Cornell University, Ithaca, NY 14853, USA
Universität des Saarlandes, 66123 Saarbrücken, Germany
Ecole Polytechnique, 91128 Palaiseau Cedex, France
Institut für Nichtlineare Dynamik, Georg-August-Universität Göttingen, 37073 Göttingen, Germany
Institut für Physik und Astronomie, Universität Potsdam, 14476 Potsdam, Germany
Identifiers:DOI:10.1186/1757-5036-3-9
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