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ID: 610794.0, MPI für Evolutionsbiologie / Experimental Evolution Research Group
Molecular quantification of Saccharomyces cerevisiae
α-pheromone secretion
Authors:Rogers, David W.; McConnell, Ellen; Greig, Duncan
Language:English
Date of Publication (YYYY-MM-DD):2012
Title of Journal:FEMS Yeast Research
Journal Abbrev.:FEMS Yeast Res
Volume:article in press
Review Status:not specified
Audience:Not Specified
Abstract / Description:Saccharomyces cerevisiae yeast cells court each other by producing an attractive
sex pheromone specific to their mating type. Cells detect the sex pheromone
from potential mates using a well-defined intracellular signalling cascade that
has become a model for studying signal transduction. In contrast, the factors
contributing to the production of pheromone itself are poorly characterized,
despite the widespread use of the S. cerevisiae a-pheromone secretion pathway
in industrial fungal protein expression systems. Progress in understanding
pheromone secretion has been hindered by a lack of a precise and quantitative
pheromone production assay. Here, we present an ELISA-based method for the
quantification of a-pheromone secretion. In the absence of pheromone from
the opposite mating type, we found that each cell secretes over 550 mature apheromone
peptides per second; 90% of this total was produced from MFa1.
The addition of a-pheromone more than doubled total a-pheromone secretion.
This technique offers several improvements on current methods for measuring
a-pheromone production and will allow detailed investigation of the factors
regulating pheromone production in yeast.
Free Keywords:yeast; signal; mating factor; ELISA
External Publication Status:published
Document Type:Article
Communicated by:Brigitte Lechner
Affiliations:MPI für Evolutionsbiologie/Max Planck Research Group Experimental Evolution
External Affiliations:Department of Genetics, Evolution, and Environment, University College London, London, UK
Identifiers:ISSN:1751-7362 (print) [ID-No:1]
ISSN:1751-7370 (online) [ID-No:2]
DOI:10.1111/j.1567-1364.2012.00817.x [ID-No:3]
LOCALID:2934/S 39279 [Listen-Nummer/S-Nummer]
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