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ID: 702243.0, MPI für Dynamik und Selbstorganisation / Hydrodynamik und Strukturbildung
Scanning X-Ray Nanodiffraction on Dictyostelium discoideum
Authors:Priebe, M; Bernhardt, M; Blum, Christoph; Tarantola, Marco; Bodenschatz, Eberhard
Language:English
Date of Publication (YYYY-MM-DD):2014-12-02
Title of Journal:Biophysics Journal
Journal Abbrev.:Biophys. J.
Volume:107
Start Page:2662
End Page:2673
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:Yes
Abstract / Description:We have performed scanning x-ray nanobeam diffraction experiments on single cells of the amoeba Dictyostelium discoideum. Cells have been investigated in 1), freeze-dried, 2), frozen-hydrated (vitrified), and 3), initially alive states. The spatially resolved small-angle x-ray scattering signal shows characteristic streaklike patterns in reciprocal space, which we attribute to fiber bundles of the actomyosin network. From the intensity distributions, an anisotropy parameter can be derived that indicates pronounced local variations within the cell. In addition to nanobeam small-angle x-ray scattering, we have evaluated the x-ray differential phase contrast in view of the projected electron density. Different experimental aspects of the x-ray experiment, sample preparation, and data analysis are discussed. Finally, the x-ray results are correlated with optical microscopy (differential phase contrast and confocal microscopy of mutant strains with fluorescently labeled actin and myosin II), which have been carried out in live and fixed states, including optical microscopy under cryogenic conditions.
External Publication Status:published
Document Type:Article
Communicated by:Folkert Müller-Hoissen
Affiliations:MPI für Dynamik und Selbstorganisation/Hydrodynamik und Strukturbildung
Identifiers:DOI:http://dx.doi.org/10.1016/j.bpj.2014.10.027
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