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          Institute: MPI für Hirnforschung     Collection: Neuroanatomy (Heinz Wässle)     Display Documents

ID: 10494.0, MPI für Hirnforschung / Neuroanatomy (Heinz Wässle)
Vesicular gamma-aminobutyric acid transporter expression in amacrine and horizontal cells
Authors:Cueva, J. G.; Haverkamp, S.; Reimer, R. J.; Edwards, R.; Wässle, H.; Brecha, N. C.
Date of Publication (YYYY-MM-DD):2002
Title of Journal:Journal of Comparative Neurology
Journal Abbrev.:J. Comp. Neurol.
Issue / Number:3
Start Page:227
End Page:237
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:The vesicular gamma-aminobutyric acid (GABA) transporter (VGAT), which transports the inhibitory amino acid transmitters GABA and glycine, is localized to synaptic vesicles in axon terminals. The localization of VGAT immunoreactivity to mouse and rat retina was evaluated with light and electron microscopy by using well-characterized VGAT antibodies. Specific VGAT immunoreactivity was localized to numerous varicose processes in all laminae of the inner plexiform layer (IPL) and to the outer plexiform layer (OPL). Amacrine cell somata characterized by weak VGAT immunoreactivity in the cytoplasm were located in the ganglion cell layer and proximal inner nuclear layer (IN-L) adjacent to the IPL. In rat retina, VGAT-immunoreactive cell bodies also contained GABA, glycine, or parvalbumin (PV) immunoreactivity, suggesting vesicular uptake of GABA or glycine by these cells. A few varicose VGAT-immunoreactive processes entered the OPL from the IPL. VGAT immunoreactivity in the OPL was predominantly localized to horizontal cell processes. VGAT and calcium binding protein-28K immunoreactivities (CaBP; a marker for horizontal cells) were colocalized in processes and terminals distributed to the OPL. Furthermore, VGAT immunoreactivity overlapped or was immediately adjacent to postsynaptic density-95 (PSD-95) immunoreactivity, which is prominent in photoreceptor terminals. Pre-embedding immunoelectron microscopy of mouse and rat retinae showed that VGAT immunoreactivity was localized to horizontal cell processes and their terminals. Immunoreactivity was distributed throughout the cytoplasm of the horizontal cell processes. Taken together, these findings demonstrate VGAT immunoreactivity in both amacrine and horizontal cell processes, suggesting these cells contain vesicles that accumulate GABA and glycine, possibly for vesicular release. (C) 2002 Wiley-Liss, Inc.
Free Keywords:retina; synaptic vesicles; GABA transport; VGAT
Comment of the Author/Creator:Date: 2002, APR 8
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Hirnforschung/Neuroanatomy (Abt.: Wässle)
External Affiliations:Univ Calif Los Angeles, Med Ctr, Dept Neurobiol, 10833 LeConte; Ave Box 951763, Los Angeles, CA 90095 USA; Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA; Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA; Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90095 USA; Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA; Univ Calif Los Angeles, CURE, Digest Dis Res Ctr, Los Angeles, CA 90095 USA; Vet Adm Greater Los Angeles Healtcare Syst, Los Angeles, CA 90073 USA
Identifiers:ISI:000174167600003 [ID No:1]
ISSN:0021-9967 [ID No:2]
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