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          Institute: MPI für Hirnforschung     Collection: Neuroanatomy (Heinz Wässle)     Display Documents



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ID: 10627.0, MPI für Hirnforschung / Neuroanatomy (Heinz Wässle)
Localization and possible function of the glutamate transporter, EAAC1, in the rat retina
Authors:Wiessner, M.; Fletcher, E. L.; Fischer, F.; Rauen, T.
Language:English
Date of Publication (YYYY-MM-DD):2002
Title of Journal:Cell and Tissue Research
Journal Abbrev.:Cell Tissue Res.
Volume:310
Issue / Number:1
Start Page:31
End Page:40
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:We investigated the localization and possible function of EAAC1 in the rat retina. Immunocytochemical localization of EAAC1 at the light-microscopic level revealed a fine dust-like labelling pattern across the two synaptic layers. Horizontal cell and subpopulations of amacrine cell somata were labelled, as were some somata within the ganglion cell layer. Some immunoreactive puncta were observed within the cytoplasm of amacrine cells, in regions well away from synaptic sites. At the ultrastructural level, EAAC1 immunolabelled one postsynaptic element at synapses and also processes well away from the synaptic release site. Since EAAC1 was localized away from synaptic sites, we evaluated the role EAAC1 plays in GABA formation by measuring GABA concentrations via reversed-phase high-performance liquid chromatography following incubation of retinae in enzyme and glutamate uptake inhibitors. Incubation of retinae in D-threo- beta-hydroxyaspartate or D/L-threo-beta-benzyloxyaspartate, which are known to inhibit the glutamate transporters GLAST1, GLT1, and EAAC1, caused a decrease in GABA synthesis by around 50%. Incubation in 6-diazo-5-OXO-L-norleucine, a phosphate- activated glutaminase inhibitor, decreased GABA formation by 40%. Taken together with the anatomical data, the results of this study suggest that EAAC1 plays very little role in GABA synthesis - indeed GABA formation occurs predominantly from glutamine. By virtue of its location both near and well away from synaptic release sites, EAAC1 may regulate glutamate uptake differentially.
Free Keywords:GABA; EAAT3; neuron; high-affinity uptake; Muller cell; rat
Comment of the Author/Creator:Date: 2002, OCT
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für Hirnforschung/Neuroanatomy (Abt.: Wässle)
External Affiliations:Univ Melbourne, Dept Optometry & Vis Sci, Cnr Keppel & Cardigan; Sts, Carlton, Vic 3053, Australia;
Identifiers:ISI:000178753100004 [ID No:1]
ISSN:0302-766X [ID No:2]
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