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          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Physical and Chemical Foundations of Process Engineering     Display Documents



ID: 116764.0, MPI für Dynamik komplexer technischer Systeme / Physical and Chemical Foundations of Process Engineering
Experimentelle und theoretische Untersuchungen der Gradientenchromatographie eines Proteins
Authors:Mihlbachler, K.; Anspach, B.; Seidel-Morgenstern, A.
Language:German
Date of Publication (YYYY-MM-DD):1998
Title of Journal:Chemie-Ingenieur-Technik
Volume:70
Issue / Number:4
Start Page:382
End Page:398
Review Status:not specified
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Preparative liquid column chromatography is a powerful method for the isolation of proteins from complex mixtures. The use of solvent gradients has proved invaluable in the optimisation of the chromatographic separation process. In contrast to isocratic chromatography, the eluting strength of the solvent is increased during the separation. To this end, an increasing proportion of a solvent of high eluting strength is added to solvent of low eluting strength. This publication is intended to contribute to the general understanding of and to a quantitative description of preparative gradient chromatography of dissolved proteins. The analysis is based on the experimental determination of various chromatograms of the model protein lysozyme on a cation exchanger.
External Publication Status:published
Document Type:Article
Communicated by:Andreas Seidel-Morgenstern
Affiliations:MPI für Dynamik komplexer technischer Systeme/Physical and Chemical Foundations of Process Engineering
Identifiers:LOCALID:12
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