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          Institute: MPI für molekulare Biomedizin     Collection: Publikationen molekulare Biomedizin     Display Documents



ID: 123149.0, MPI für molekulare Biomedizin / Publikationen molekulare Biomedizin
Angiogenic capacity of endothelial cells in islets of Langerhans
Authors:Linn, T.; Schneider, K.; Hammes, H. P.; Preissner, K. T.; Brandhorst, H.; Morgenstern, E.; Kiefer, F.; Bretzel, R. G.
Language:English
Date of Publication (YYYY-MM-DD):2003-05
Title of Journal:FASEB Journal
Journal Abbrev.:FASEB J
Volume:17
Issue / Number:8
Start Page:881
End Page:883
Review Status:not specified
Audience:Not Specified
Abstract / Description:Transplantation of pancreatic islets reconstitutes glucose homeostasis in diabetes mellitus. Before transplantation, islets are disrupted from the surrounding blood vessels by the isolation procedure, with the grafted tissue being subject to ischemic damage. The survival of transplanted islets is assumed to depend on effective revascularization. Perfusion studies suggest that newly formed microvessels supplying the graft with nutrients are exclusively rebuilt by the host. It is generally not known whether isolated islets contain endothelial cells (EC), which potentially participate in the revascularization process. Therefore, we tried to detect immature EC in isolated islets by transformation with polyoma middle T antigen. Endothelioma cells were generated, implicating the presence of de-differentiated EC within isolated islets. When embedded in a fibrin gel, the islets developed cellular cords consisting of EC, whereas FGF-2 and VEGF stimulated the formation of cord-like structures. Furthermore, we studied the presence of donor EC in islet grafts by using transgenic mice with an EC lineage-specific promotor-LacZ reporter construct (Tie-2LacZ). Following islet transplantation, Tie-2LacZ-positive EC of both donor and recipient were identified in the vicinity of or within the graft up to 3 wk after transplantation. In conclusion, EC and/or their progenitors with angiogenic capacity reside within isolated islets of different species, and their proliferative potential can be stimulated by various inducers. These graft-related endothelia persist after islet transplantation and are integrated within newly formed microvessels.
Free Keywords:Animals
; Cell Division/drug effects
; Cell Hypoxia/physiology
; Endothelial Growth Factors/pharmacology
; Endothelium, Vascular/*cytology/drug effects/physiology
; Fibroblast Growth Factors/pharmacology
; Glucose/pharmacology
; Human
; Intercellular Signaling Peptides and Proteins/pharmacology
; Islets of Langerhans/blood supply/*cytology/metabolism
; Islets of Langerhans Transplantation
; Lac Operon/genetics
; Lymphokines/pharmacology
; Mice
; Mice, Transgenic
; Neovascularization, Physiologic/drug effects/*physiology
; Rats
; Tumor Necrosis Factor/pharmacology
; Vascular Endothelial Growth Factor A
; Vascular Endothelial Growth Factors
; von Willebrand Factor/metabolism
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Biomedizin
Identifiers:URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:1]
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