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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



ID: 124922.0, MPI für medizinische Forschung / Abteilung Biophysik
Electron cryo-microscopy shows how strong binding of myosin to actin releases nucleotide
Authors:Holmes, Kenneth C.; Angert, Isabel; Kull, F. John; Jahn, Werner; Schröder, Rasmus R.
Language:English
Date of Publication (YYYY-MM-DD):2003-09-25
Title of Journal:Nature
Journal Abbrev.:Nat.
Volume:425
Start Page:423
End Page:427
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Muscle contraction involves the cyclic interaction of the myosin cross-bridges with the actin filament, which is coupled to steps in the hydrolysis of ATP1. While bound to actin each cross-bridge undergoes a conformational change, often referred to as the power stroke, which moves the actin filament past the myosin filaments; this is associated with the release of the products of ATP hydrolysis and a stronger binding of myosin to actin. The association of a new ATP molecule weakens the binding again, and the attached cross-bridge rapidly dissociates from actin. The nucleotide is then hydrolysed, the conformational change reverses, and the myosin cross-bridge reattaches to actin. X-ray crystallography has determined the structural basis of the power stroke, but it is still not clear why the binding of actin weakens that of the nucleotide and vice versa. Here we describe, by fitting atomic models of actin and the myosin cross-bridge into high-resolution electron cryo-microscopy three-dimensional reconstructions, the molecular basis of this linkage. The closing of the actin-binding cleft when actin binds is structurally coupled to the opening of the nucleotide-binding pocket.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik
Relations:-URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=4258719&dopt=Abstract
-URI:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=10872464&dopt=Abstract
Identifiers:URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Abstract HTML]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Fulltext HTML]
URI:http://www.nature.com/cgi-taf/DynaPage.taf?file=/n... [Fulltext PDF]
DOI:10.1038/nature02005
LOCALID:5999 [PUMA publication management ID]
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