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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomolekulare Mechanismen     Display Documents



ID: 125115.0, MPI für medizinische Forschung / Abteilung Biomolekulare Mechanismen
Structure of Rab GDP-Dissociation Inhibitor in Complex with Prenylated YPT1 GTPase
Authors:Rak, Alexey; Pylypenko, Olena; Durek, Thomas; Watzke, Anja; Kushnir, Susanna; Brunsveld, Lucas; Waldmann, Herbert; Goody, Roger S.; Alexandrov, Kirill
Language:English
Date of Publication (YYYY-MM-DD):2003-10-24
Title of Journal:Science
Volume:302
Issue / Number:5645
Start Page:446
End Page:650
Review Status:not specified
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the guanosine diphosphate (GDP) dissociation inhibitor (RabGDI). Using a combination of chemical synthesis and protein engineering, we generated and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of the complex was solved to 1.5 angstrom resolution and provides a structural basis for the ability of RabGDI to inhibit the release of nucleotide by Rab proteins. Isoprenoid binding requires a conformational change that opens a cavity in the hydrophobic core of its domain II. Analysis of the structure provides a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans.
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
Relations:-URI:http://www.jbc.org/cgi/content/full/279/12/11480
-URI:http://www.ejbiochem.org/cgi/content/full/271/4/663
Identifiers:URI:http://www.sciencemag.org/cgi/content/abstract/302... [Abstract HTML]
URI:http://www.sciencemag.org/cgi/content/full/302/564... [Fulltext HTML]
URI:http://www.sciencemag.org/cgi/reprint/302/5645/646 [Fulltext PDF]
DOI:10.1126/science.1087761
LOCALID:6085 [PUMA publication management ID]
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