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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 126737.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
A cryptic plasmid of Yersinia enterocolitica encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil
Authors:Strauch, Eckhard; Goelz, Greta; Knabner, Dorethea; Konietzny, Antje; Lanka, Erich; Appel, Bernd
Language:English
Date of Publication (YYYY-MM-DD):2003-10
Title of Journal:Microbiology-SGM
Journal Abbrev.:Microbiology-(UK)
Volume:149
Issue / Number:10
Start Page:2829
End Page:2845
Copyright:Received 11 April 2003; revised 16 June 2003; accepted 25 June 2003.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Yersinia enterocolitica 29930 (biotype 1A; O : 7,8), the producing strain of the phage-tail-like bacteriocin enterocoliticin, possesses a plasmid-encoded conjugative type IV transfer system. The genes of the conjugative system were found by screening of a cosmid library constructed from total DNA of strain 29930. The cosmid Cos100 consists of the vector SuperCos1 and an insert DNA of 40 303 bp derived from a cryptic plasmid of strain 29930. The conjugative transfer system consists of genes encoding a DNA transfer and replication system (Dtr) with close relationship to the mob region of the mobilizable plasmid CloDF13 and a gene cluster encoding a mating pair formation system (Mpf) closely related to the Mpf system of the IncX plasmid R6K. However, a gene encoding a homologue of TaxB, the coupling protein of the IncX system, is missing. The whole transfer region has a size of approximately 17 kb. The recombinant plasmid Cos100 was shown to be transferable between Escherichia coli and Yersinia with transfer frequencies up to 0·1 transconjugants per donor. Mutations generated by inserting a tetracycline cassette into putative tri genes yielded a transfer-deficient phenotype. Conjugative transfer of the cryptic plasmid could not be demonstrated in the original host Y. enterocolitica 29930. However, a kanamycin-resistance-conferring derivative of the plasmid was successfully introduced into E. coli K-12 by transformation and was shown to be self-transmissible. Furthermore, Southern blot hybridization and PCR experiments were carried out to elucidate the distribution of the conjugative transfer system in Yersinia. In total, six Y. enterocolitica biotype 1A strains harbouring closely related systems on endogenous plasmids were identified.
Comment of the Author/Creator:Received 11 April 2003; revised 16 June 2003; accepted 25 June 2003.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Robert Koch Inst, Projekt Horizontaler Gentransfer, D-13353 Berlin, Germany.; Max Planck Inst Mol Genet, D-14195 Berlin, Germany.
Identifiers:ISI:000186008200015 [ID No:1]
ISSN:1350-0872 [ID No:2]
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