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          Institute: MPI für molekulare Physiologie     Collection: Abteilung III - Physikalische Biochemie     Display Documents



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ID: 12719.0, MPI für molekulare Physiologie / Abteilung III - Physikalische Biochemie
Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes
Authors:Rothwell, Paul James; Berger, Sylvia; Kensch, Oliver; Felekyan, Suren; Antonik, Matthew; Wöhrl, Birgitta Maria; Restle, Tobias; Goody, Roger S.; Seidel, Claus A. M.
Language:English
Research Context:HIV proteins
Date of Publication (YYYY-MM-DD):2003-02-18
Title of Journal:Proceedings of the National Academy of Sciences of the USA
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:100
Issue / Number:4
Start Page:1655
End Page:1660
Sequence Number of Article:1
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:By using single-molecule multiparameter fluorescence detection, fluorescence resonance energy transfer experiments, and newly developed data analysis methods, this study demonstrates directly the existence of three structurally distinct forms of reverse transcriptase (RT):nucleic acid complexes in solution. Single-molecule multiparameter fluorescence detection also provides first information on the structure of a complex not observed by x-ray crystallography. This species did not incorporate nucleotides and is structurally distinct from the other two observed species. We determined that the nucleic acid substrate is bound at a site far removed from the nucleic acid- binding tract observed by crystallography. In contrast, the other two states are identified as being similar to the x-ray crystal structure and represent distinct enzymatically productive stages in DNA polymerization. These species differ by only a 5-Angstrom shift in the position of the nucleic acid. Addition of nucleoside triphosphate or of inorganic pyrophosphate allowed us to assign them as the educt and product state in the polymerization reaction cycle; i.e., the educt state is a complex in which the nucleic acid is positioned to allow nucleotide incorporation. The second RT:nucleic acid complex is the product state, which is formed immediately after nucleotide incorporation, but before RT translates to the next nucleotide.
External Publication Status:published
Document Type:Article
Affiliations:MPI für molekulare Physiologie/Abteilung III - Physikalische Biochemie/AG HIV-Proteine: Prof. Dr. Roger Goody
MPI für molekulare Physiologie/Abteilung III - Physikalische Biochemie/AG Biochemie viraler Polymerasen: PD Dr. Tobias Restle
MPI für biophysikalische Chemie/Abt. Jürgen Troe / 010
Identifiers:URL:http://dx.doi.org/10.1073/pnas.0434003100 [full text of this article]
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