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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. of Biochemistry (Klaus Hahlbrock)     Display Documents



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ID: 127496.0, MPI für Pflanzenzüchtungsforschung / Dept. of Biochemistry (Klaus Hahlbrock)
Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley
Authors:Cormack, R. S.; Eulgem, T.; Rushton, P. J.; Koechner, P.; Hahlbrock, K.; Somssich, I. E.
Language:English
Date of Publication (YYYY-MM-DD):2002-06-07
Title of Journal:Biochimica et Biophysica Acta-Gene Structure and Expression
Journal Abbrev.:Biochim. Biophys. Acta-Gene Struct. Expression
Volume:1576
Issue / Number:1-2
Start Page:92
End Page:100
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one- hybrid system. In yeast, both proteins interacted sequence- specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo- binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene, the first parsley member of the group III family of WRKY proteins, is extremely transient, with high mRNA levels occurring within a time window of less than I h. WRKY4 and -5, as well as the previously identified parsley transcription factors WRKY 1 and -3, are encoded by immediate early elicitor-activated genes that differ in their sensitivity to cycloheximide (CHX) and their activation kinetics. We propose that a number of the pathways activated during the plant defense response require the induction of several distinct WRKY transcription factors with different DNA binding- site preferences to fine-tune the activation of a wide spectrum of target genes. (C) 2002 Elsevier Science B.V. All rights reserved.
Free Keywords:protein synthesis inhibitor study; transient transfection assay; yeast one-hybrid screen; WRKY transcription factor
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Max Planck Inst Zuchtungsforsch, Biochem Abt, Carl Von Linne; Weg 10, D-50829 Cologne, Germany; Max Planck Inst Zuchtungsforsch, Biochem Abt, D-50829 Cologne, Germany
Identifiers:ISI:000176060700011 [ID No:1]
ISSN:0167-4781 [ID No:2]
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