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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents

ID: 127563.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Transcriptome analysis of sulfur depletion in Arabidopsis thaliana: interlacing of biosynthetic pathways provides response specificity
Authors:Nikiforova, V.; Freitag, J.; Kempa, S.; Adamik, M.; Hesse, H.; Hoefgen, R.
Date of Publication (YYYY-MM-DD):2003
Title of Journal:Plant Journal
Issue / Number:4
Start Page:633
End Page:650
Review Status:not specified
Audience:Not Specified
Abstract / Description:Higher plants assimilate inorganic sulfate into cysteine, which is subsequently converted to methionine, and into a variety of other sulfur-containing organic compounds. To resist sulfur deficiency, plants must demonstrate physiological flexibility: the expression of an extensive set of genes and gene regulators that act in the affected pathways or signalling cascades must be delicately tuned in response to environmental challenges. To elucidate this network of interactions, we have applied an array hybridisation/transcript profiling method to Arabidopsis plants subjected to 6, 10 and 13 days of constitutive and induced sulfur starvation. The temporal expression behaviour of approximately 7200 non-redundant genes was analysed simultaneously. The experiment was designed in a way to identify statistically significant changes of gene expression based on sufficient numbers of repeated hybridisations performed with five uniform pools of plant material. The expression profiles were processed to select differentially expressed genes. Among the 1507 sulfur-responsive clones implicated in this way, 632 genes responded specifically to sulfur deficiency by significant over-expression. The sulfur-responsive genes were grouped according to functional categories or biosynthetic pathways. As expected, genes of the sulfur assimilation pathway were altered in expression. Furthermore, genes involved in flavonoid, auxin, and jasmonate biosynthesis pathways were upregulated in conditions of sulfur deficiency. Based on the correlative analysis of gene expression patterns, we suggest that a complex co-ordination of systematic responses to sulfur depletion is provided via integration of flavonoid, auxin and jasmonate pathway elements. Plait concept for transduction of specificity via the main non-specific signalling stream is proposed. [References: 77] 77
Free Keywords:R2r3-myb gene family. Jasmonic acid. Sulfate transporter. Microarray
; analysis. Auxin-transport. Plant defense. Phenylpropanoid
; biosynthesis. Deficiency stress. Oxidative stress. Cdna microarray.
; Plant Sciences in Current Contents(R)/Agricultural, Biology &
; Environmental Sciences
; Animal & Plant Sciences in Current Contents(R)/Life Sciences.
; 2003 week 13
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Hoefgen
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