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          Institute: MPI für neurologische Forschung     Collection: Emeritus-Collection Prof. Hossmann     Display Documents



ID: 13137.0, MPI für neurologische Forschung / Emeritus-Collection Prof. Hossmann
Monitoring of implanted stem cell migration in vivo: A highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat
Authors:Hoehn, Mathias; Küstermann, Ekkehard; Blunk, James; Wiedermann, Dirk; Trapp, Thorsten; Wecker, Stefan; Föcking, Melanie; Arnold, Heinz; Hescheler, J.; Fleischmann, B. K.; Schwindt, Wolfram; Bührle, Christian
Language:English
Date of Publication (YYYY-MM-DD):2002-12-10
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:99
Issue / Number:25
Start Page:16267
End Page:16272
Copyright:Copyright 2002 by the National Academy of Sciences of the United States of America, all rights reserved
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:In vivo monitoring of stem cells after grafting is essential for a better understanding of their migrational dynamics and differentiation processes and of their regeneration potential. Migration of endogenous or grafted stem cells and neurons has been described in vertebrate brain, both under normal conditions from the subventricular zone along the rostral migratory stream and under pathophysiological conditions, such as degeneration or focal cerebral ischemia. Those studies, however, relied on invasive analysis of brain sections in combination with appropriate staining techniques. Here, we demonstrate the observation of cell migration under in vivo conditions, allowing the monitoring of the cell dynamics within individual animals, and for a prolonged time. Embryonic stem (ES) cells, constitutively expressing the GFIP, were labeled by a lipofection procedure with a MRI contrast agent and implanted into rat brains. Focal cerebral ischemia had been induced 2 weeks before implantation of ES cells into the healthy, contralateral hemisphere. MRI at 78-mum isotropic spatial resolution permitted the observation of the implanted cells with high contrast against the host tissue, and was confirmed by GFP registration. During 3 weeks, cells migrated along the corpus callosum to the ventricular walls, and massively populated the borderzone of the damaged brain tissue on the hemisphere opposite to the implantation sites. Our results indicate that ES cells have high migrational dynamics, targeted to the cerebral lesion area. The imaging approach is ideally suited for the noninvasive observation of cell migration, engraftment, and morphological differentiation at high spatial and temporal resolution.
Free Keywords:embryonic stem cells; cerebral ischemia; cell labeling
Comment of the Author/Creator:Date: 2002, DEC 10
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für neurologische Forschung/Experimentelle Neurologie - Abt. Prof. Hossmann/Arbeitsgruppe Prof. Hoehn (NMR)
External Affiliations:Max Planck Inst Neurol Res, Gleueler Str 50, D-50931 Cologne,; Germany; Max Planck Inst Neurol Res, D-50931 Cologne, Germany; Univ Cologne, Dept Neurophysiol, D-50937 Cologne, Germany
Identifiers:ISI:000179783400080 [ID No:1]
ISSN:0027-8424 [ID No:2]
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