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          Institute: MPI für marine Mikrobiologie     Collection: Arbeitsgruppe Mikrosensorik     Display Documents

ID: 13864.0, MPI für marine Mikrobiologie / Arbeitsgruppe Mikrosensorik
Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat
Authors:Grötzschel, S.; Köster, J.; Abed, R. M. M.; de Beer, D.
Date of Publication (YYYY-MM-DD):2002-08
Title of Journal:Biodegradation
Journal Abbrev.:Biodegradation
Issue / Number:4
Start Page:273
End Page:283
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In this study the degradation of hydrophobic petroleum model compounds ( phenanthrene, pristane, octadecane and dibenzothiophene) added to a submersed hypersaline microbial mat was investigated. Montmorillonite with an artificially altered, hydrophobic surface was used as carrier material, forming an organo-clay complex (OCC) with the attached mixture of petroleum model compounds. 6 mg/cm(2) OCC were applied to cyanobacterial mat pieces, containing similar to33.3 mug/mg OCC of each compound. The degradation experiment was performed under controlled laboratory conditions and accompanied by chemical analyses by GC/GC-MS, molecular analyses by PCR and DGGE as well as functional analyses by microsensor measurements of oxygen, photosynthesis, sulfide, pH and light. All applied model compounds were degraded, but residues were still present after 18 weeks. The aromatic compounds phenanthrene (5.1 mug/mg OCC) and dibenzothiophene (4.3 mug/mg OCC) were preferentially degraded compared to the alkanes pristane (12.4 mug/mg OCC) and n-octadecane (13.4 mug/mg OCC). Metabolic changes during the degradation process could not be detected by microsensor measurements. The molecular population analyses did not reveal any significant community changes concomitant with the decrease of the petroleum model compounds. We conclude, that the pristine mats represent an intact, robust ecosystem in which the enzymatic requirements for the degradation of the applied pollutants exist. The slow degradation process did not affect the usual high internal turnover rates and did not favor a certain population in the community of the mats.
Free Keywords:cyanobacteria; dibenzothiophene; microsensors; octadecane; PCR- DGGE; phenanthrene; pristane
Comment of the Author/Creator:Date: 2002, AUG
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für marine Mikrobiologie
External Affiliations:Max Planck Inst Marine Microbiol, Celsiusstr 1, D-28359 Bremen,; Germany; Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany; Inst Chem & Biol Marine Environm, D-26111 Oldenburg, Germany
Identifiers:ISI:000179496400006 [ID No:1]
ISSN:0923-9820 [ID No:2]
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