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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents



ID: 15359.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans
Authors:Hannak, E.; Kirkham, M.; Hyman, A. A.; Oegema, K.
Language:English
Date of Publication (YYYY-MM-DD):2001-12-24
Title of Journal:Journal of Cell Biology
Journal Abbrev.:J. Cell Biol.
Volume:155
Issue / Number:7
Start Page:1109
End Page:1115
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Centrosomes mature as cells enter mitosis, accumulating gamma - tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA- mediated interference (RNAi) to examine the role of the aurora- A kinase, AlR-1, during centrosome maturation in Caenorhabditis elegans. In air-1(RNAi) embryos, centrosomes separate normally, an event that occurs before maturation in C. elegans. After nuclear envelope breakdown, the separated centrosomes collapse together, and spindle assembly fails. In mitotic air-1(RNAi) embryos, centrosomal alpha -tubulin fluorescence intensity accumulates to only 40% of wild-type levels, suggesting a defect in the maturation process. Consistent with this hypothesis, we find that AIR-1 is required for the increase in centrosomal gamma -tubulin and two other PCM components, ZYG-9 and CeGrip, as embryos enter mitosis. Furthermore, the AIR-1- dependent increase in centrosomal gamma -tubulin does not require MTs. These results suggest that aurora-A kinases are required to execute a MT-independent pathway for the recruitment of PCNA during centrosome maturation.
Free Keywords:microtubule; mitosis; cell cycle; cancer
Comment of the Author/Creator:Date: 2001, DEC 24
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
External Affiliations:MPI Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden,; Germany; Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany; European Mol Biol Lab, D-69117 Heidelberg, Germany
Identifiers:ISI:000173023500007 [ID No:1]
ISSN:0021-9525 [ID No:2]
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