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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents



ID: 15392.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
Single proteins observed by atomic force microscopy
Authors:Scheuring, S.; Fotiadis, D.; Möller, C.; Müller, S. A.; Engel, A.; Müller, D. J.
Language:English
Date of Publication (YYYY-MM-DD):2001
Title of Journal:Single Molecules
Journal Abbrev.:Single Mol.
Volume:2
Issue / Number:2
Start Page:59
End Page:67
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Here we discuss the experimental approaches that have allowed high resolution atomic force microscopy (AFM) imaging, and review results that show AFM to be of great interest for biologists, AFM allows single proteins to be imaged under physiologically relevant conditions. The exceptional signal-to- noise ratio and resolution of AFM topographs enables the oligomerization state and characteristic substructures of individual proteins to be resolved. Several examples demonstrate the capabilities of AFM to directly observe single proteins, and their conformational changes, to study protein- protein interactions and to follow the assembly of membrane proteins. We consider the AFM techniques that have allowed high resolution imaging, and review results that show AFM to be a powerful method to analyze biological processes at the level of single molecules.
Comment of the Author/Creator:Date: 2001
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
External Affiliations:Max Planck Inst Mol Cell Biol & Genet, ME Muller Inst Struct; Biol, D-01307 Dresden, Germany; Max Planck Inst Mol Cell Biol & Genet, ME Muller Inst Struct Biol, D-01307 Dresden, Germany; Univ Basel, Biozentrum, ME Muller Inst Struct Biol, CH-4056 Basel, Switzerland; KFA Julich GmbH, Forschungszentrum, D-52425 Julich, Germany
Identifiers:ISI:000174074500002 [ID No:1]
ISSN:1438-5163 [ID No:2]
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