Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents



  history
ID: 15475.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
XMAP215 is required for the microtubule-nucleating activity of centrosomes
Authors:Popov, A. V.; Severin, F.; Karsenti, E.
Language:English
Date of Publication (YYYY-MM-DD):2002-08-06
Title of Journal:Current Biology
Journal Abbrev.:Curr. Biol.
Volume:12
Issue / Number:15
Start Page:1326
End Page:1330
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Microtubules are essential structures that organize the cytoplasm and form the mitotic spindle. Their number and orientation depend on the rate of nucleation events and their dynamics. Microtubules are often, but not always, nucleated off a single cytoplasmic element, the centrosome. One microtubule- associated protein, XMAP215 [1], is also a resident centrosomal protein [2]. In this study, we have found that XMAP215 is a key component for the microtubule-nucleating activity of centrosomes. We show that depletion of XMAP215 from Xenopus egg extracts impairs their ability to reconstitute the microtubule nucleation potential of salt-stripped centrosomes. We also show that XMAP215 immobilized on polymer beads induces the formation of microtubule asters in egg extracts as well as in solutions of pure tubulin. Formation of asters by XMAP215 beads indicates that this protein is able to anchor nascent microtubules via their minus ends. The aster-forming activity of XMAP215 does not require gamma-tubulin in pure tubulin solutions, but it is gamma-tubulin-dependent in egg extracts. Our results indicate that XMAP215, a resident centrosomal protein, contributes to the microtubule-nucleating activity of centrosomes, suggesting that, in vivo, the formation of asters by centrosomes requires factors additional to gamma-tubulin.
Comment of the Author/Creator:Date: 2002, AUG 6
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
External Affiliations:European Mol Biol Lab, Cell Biol Program, D-69117 Heidelberg, Germany;
Identifiers:ISI:000177358100021 [ID No:1]
ISSN:0960-9822 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.