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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents



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ID: 15478.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
Sampling effects influence heights measured with atomic force microscopy
Authors:Heymann, J. B.; Möller, C.; Müller, D. J.
Language:English
Date of Publication (YYYY-MM-DD):2002-07
Title of Journal:Journal of Microscopy-Oxford
Journal Abbrev.:J. Microsc.-Oxf.
Volume:207
Start Page:43
End Page:51
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:The atomic force microscope (AFM) is an exquisitely delicate probe measuring the height of a specimen at discrete sampling points in a fixed two-dimensional (2D) raster. The resulting topograph is a 2D digital image, with each pixel representing a distinct height measurement. The height of an object is determined as the average of the maximum heights measured above the supporting surface. We show that such object heights derived from a variety of organic samples depend critically on the sampling or pixel size of the 2D raster. It is concluded that to obtain accurate specimen heights, the pixel size must be small enough to resolve submolecular structures and thus ensure representative sampling of the height variation on the surface.
Free Keywords:AFM; Nyquist theorem; membrane; protein; resolution
Comment of the Author/Creator:Date: 2002, JUL
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
External Affiliations:NIAMS, LSBR, NIH, Bethesda, MD 20892, USA; Tech Univ Dresden, BIOTEC, Dresden, Germany
Identifiers:ISI:000177026900005 [ID No:1]
ISSN:0022-2720 [ID No:2]
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