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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents

ID: 15621.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
Rvs161p and sphingolipids are required for actin repolarization following salt stress
Authors:Balguerie, A.; Bagnat, M.; Bonneu, M.; Aigle, M.; Breton, A. M.
Date of Publication (YYYY-MM-DD):2002-12
Title of Journal:Eukaryotic Cell
Journal Abbrev.:Eukaryot. Cell
Issue / Number:6
Start Page:1021
End Page:1031
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In Saccharomyces cerevisiae, the actin cytoskeleton is depolarized by NaCl stress. In this study, the response was maximal after 30 min, and then actin patches repolarized. Rvs161p was required for actin repolarization because the rvs161Delta mutant did not repolarize actin patches after growth in a salt medium. Mutations suppressing the rvs161Delta- related salt sensitivity all occurred in genes required for sphingolipid biosynthesis: FEN1, SUR4, SUR2, SUR1, and IPT1. These suppressors also suppressed act1-1-related salt sensitivity and the defect in actin repolarization of the rvs161Delta mutant, providing a link between sphingolipids and actin polarization. Indeed, deletion of the suppressor genes suppressed the rvs161Delta defect in actin repolarization in two ways: either actin was not depolarized at the wild-type level in a set of suppressor mutants, or actin was repolarized in the absence of Rvs161p in the other suppressor mutants. Rvs161p was localized as cortical patches that concentrated at polarization sites, i.e., bud emergence and septa, and was found to be associated with lipid rafts. An important link between sphingolipids and actin polarization is that Rvs161p was required for actin repolarization and was found to be located in lipid rafts.
Comment of the Author/Creator:Date: 2002, DEC
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
External Affiliations:IBGC, CNRS UMR 5095, 1 Rue Camille St Saens, F-33077 Bordeaux, France;
Identifiers:ISI:000179722200018 [ID No:1]
ISSN:1535-9778 [ID No:2]
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