Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



  history
ID: 16381.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
Maternal induction of ventral fate by zebrafish radar
Authors:Sidi, S.; Goutel, C.; Peyrieras, N.; Rosa, F. M.
Language:English
Date of Publication (YYYY-MM-DD):2003-03-18
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Volume:100
Issue / Number:6
Start Page:3315
End Page:3320
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In vertebrate embryos, maternal determinants are thought to preestablish the dorsoventral axis by locally activating zygotic ventral- and dorsal-specifying genes, e.g., genes encoding bone morphogenetic proteins (BMPs) and BMP inhibitors, respectively. Whereas the canonical Wnt/beta-catenin pathway fulfills this role dorsally, the existence of a reciprocal maternal ventralizing signal remains hypothetical. Maternal noncanonical Wnt/Ca2+ signaling promote ventral fates by suppressing Wnt/beta-catenin dorsalizing signals; however, whether any maternal determinant is directly required for the activation of zygotic ventral-specifying genes is unknown. Here, we show that such a function is achieved, in part, in the zebrafish embryo by the maternally encoded transforming growth factor beta (TGF-beta) signaling molecule, Radar. Loss-of- function experiments, together with epistasis analyses, identify maternal Radar as an upstream activator of bmps expression. Maternal induction of bmps by Radar is essential for zebrafish development as its removal results in larval- lethal dorsalized phenotypes. Double-morphant analyses further suggest that Radar functions through the TGF-beta receptor Alk8 to initiate the expression of bmp genes. Our results support the existence of a previously uncharacterized maternal ventralizing pathway. They might further indicate that maternal TGF-beta/Rdr and Wnt/Ca2+ pathways complementarily specify ventral cell fates, with the former triggering bmps expression and the latter indirectly repressing genes encoding BMP antagonists.
Comment of the Author/Creator:Date: 2003, MAR 18
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
External Affiliations:Ecole Normale Super, INSERM, U368, F-75005 Paris, France; Ecole Normale Super, INSERM, U368, F-75005 Paris, France; Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany
Identifiers:ISI:000181675200061 [ID No:1]
ISSN:0027-8424 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.