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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 2 (bis 2006) - Uli Schwarz     Display Documents

ID: 16411.0, MPI für Entwicklungsbiologie / Abteilung 2 (bis 2006) - Uli Schwarz
Chemical composition of the attachment pad secretion of the locust Locusta migratoria
Authors:Votsch, W.; Nicholson, G.; Muller, R.; Stierhof, Y. D.; Gorb, S.; Schwarz, U.
Date of Publication (YYYY-MM-DD):2002-12
Title of Journal:Insect Biochemistry and Molecular Biology
Journal Abbrev.:Insect Biochem. Mol. Biol.
Issue / Number:12
Start Page:1605
End Page:1613
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:This study is the first attempt to characterise the chemical composition of the secretion of the smooth pads of the locust Locusta migratoria and to relate this to the composition of the cuticle coverage of the pads and the wings. Gas-chromatography and mass-spectrometry (GC-MS) were the principal techniques used for the characterization of these materials. Secretion droplets were visualised and quantified with the aid of diverse microscopic techniques. The chemical composition of prints is shown to differ from the cuticle coverage, in particular, with respect to the fatty acid distribution: in the secretion, saturated and unsaturated fatty acids with chain lengths between C-16 and C-20 in both the free form and as glycerides predominate, whereas cuticle coverage contains waxes of long- chained fatty-acids bound to long-chain primary alcohols. The second important difference is the significant amount of glucose and other saccharides found in methanolyzates of the pad fluid. A considerable amount of the amino acids (up to 53%) was detected in the non-volatile portion of the fluid. Data obtained from the shock-freezing, carbon-platinum coating and replica preparation show that the secretory droplets contain nano-droplets on their surfaces. The results lead us to suggest that the pad secretion is an emulsion consisting of lipidic nano-droplets dispersed in an aqueous liquid. According to the chemical composition of the secretion, a high-viscosity of the fluid may be suggested. Presumably, the fluid is a kind of a coupling agent, promoting and strengthening adhesion between otherwise incompatible materials by providing the proximity of contact for intermolecular forces. (C) 2002 Elsevier Science Ltd. All rights reserved.
Free Keywords:cuticle; attachment devices; adhesion; secretion; chemical composition; euplantulae; TLC; GC-MS; locust; Insecta; Orthoptera; Locusta migratoria
Comment of the Author/Creator:Date: 2002, DEC
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 2 - Biochemie (Uli Schwarz)
External Affiliations:Max Planck Inst Met Res, D-75069 Stuttgart, Germany; Univ Tubingen, ZMBP Zentrum Mol Biol Pflanzen, D-72076 Tubingen, Germany; Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
Identifiers:ISI:000179694100002 [ID No:1]
ISSN:0965-1748 [ID No:2]
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