Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für biophysikalische Chemie     Collection: Biochemie und Zellbiologie (Prof. Klaus Weber)     Display Documents



  history
ID: 16474.0, MPI für biophysikalische Chemie / Biochemie und Zellbiologie (Prof. Klaus Weber)
Analysis of gene function in somatic mammalian cells using small interfering RNAs
Authors:Elbashir, S. M.; Harborth, J.; Weber, K.; Tuschl, T.
Language:English
Date of Publication (YYYY-MM-DD):2002-02
Title of Journal:Methods
Volume:26
Issue / Number:2
Start Page:199
End Page:213
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:RNA interference (RNAi) is a highly conserved gene silencing mechanism that uses double-stranded RNA (dsRNA) as a signal to trigger the degradation of homologous mRNA. The mediators of sequence-specific mRNA degradation are 21- to 23-nt small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Twenty-one-nuclcotide siRNA duplexes trigger specific gene silencing in mammalian somatic cells without activation of the unspecific interferon response. Here we provide a collection of protocols for siRNA-mediated knockdown of mammalian gene expression. Because of the robustness of the siRNA knockdown technology. genomewide analysis of human gene function in cultured cells has now become possible. (C) 2002 Elsevier Science (USA). All rights reserved.
Free Keywords:RNA interference; small interfering RNA; posttranscriptional gene silencing; knockdown; double-stranded RNA
Comment of the Author/Creator:Date: 2002, FEB
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Klaus Weber / 090
MPI für biophysikalische Chemie/AG Thomas Tuschl
Identifiers:URL:http://www.sciencedirect.com/science?_ob=MImg&_ima...
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.