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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für biophysikalische Chemie     Collection: Ehemalige Abteilungen     Display Documents   history ID: 16483.0, MPI für biophysikalische Chemie / Ehemalige Abteilungen ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat Authors: Rein, U.; Andag, U.; Duden, R.; Schmitt, H. D.; Spang, A. Language: English Date of Publication (YYYY-MM-DD): 2002-04-29 Title of Journal: Journal of Cell Biology Volume: 157 Issue / Number: 3 Start Page: 395 End Page: 404 Review Status: Peer-review Audience: Not Specified Abstract / Description: In eukaryotic cells, secretion is achieved by vesicular transport. Fusion of such vesicles with the correct target compartment relies on SNARE proteins on both vesicle (v-SNARE) and the target membranes (t-SNARE). At present it is not clear how v-SNAREs are incorporated into transport vesicles. Here, we show that binding of ADP-ribosylation factor (ARF)-GTPase- activating protein (GAP) to ER-Golgi v-SNAREs is an essential step for recruitment of Arf1p and coatomer, proteins that together form the COPI coat. ARF-GAP acts catalytically to recruit COPI components. Inclusion of v-SNAREs into COPI vesicles could be mediated by direct interaction with the coat. The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding. Free Keywords: Arf; ARF-GAP; COPI; ER-Golgi SNAREs; protein transport Comment of the Author/Creator: Date: 2002, APR 29 External Publication Status: published Document Type: Article Communicated by: N. N. Affiliations: MPI für biophysikalische Chemie/AG Hans-Dieter Schmitt External Affiliations: Friedrich Miescher Lab, Max Planck Soc, D-72076 Tubingen, Germany; Univ Cambridge, Wellcome Trust Ctr Mol Mechanisms Dis, Cambridge CB2 2XY, England Identifiers: URL:http://jcb.rupress.org/content/157/3/395.full.pdf+... The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.