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          Institute: MPI für biophysikalische Chemie     Collection: NanoBiophotonik (Dr. Stefan Hell)     Display Documents



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ID: 16487.0, MPI für biophysikalische Chemie / NanoBiophotonik (Dr. Stefan Hell)
Determination of the unknown phase difference in 4Pi-confocal microscopy through the image intensity
Authors:Blanca, C. M.; Bewersdorf, J.; Hell, S. W.
Language:English
Date of Publication (YYYY-MM-DD):2002-06-01
Title of Journal:Optics Communications
Volume:206
Issue / Number:4-6
Start Page:281
End Page:285
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:We investigate the effect of the phase difference of the interfering wavefronts on the image intensity in 4Pi-confocal microscopy and thereby introduce a method to diminish the problem of the unknown phase difference in this microscope. An efficient implementation of our method utilizes binary amplitude filters reshaping the point-spread-function such that a significant disparity arises between the maximum fluorescence intensity in the constructive and destructive interference case. In the presence of planar and point-like features in the sample this property enables the system to unambiguously determine the mode of interference. (C) 2002 Elsevier Science B.V. All rights reserved
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
Identifiers:URL:http://www.sciencedirect.com/science?_ob=MImg&_ima...
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