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          Institute: MPI für biophysikalische Chemie     Collection: Neurobiologie (Prof. Reinhard Jahn)     Display Documents

ID: 16640.0, MPI für biophysikalische Chemie / Neurobiologie (Prof. Reinhard Jahn)
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules
Authors:Riedel, D.; Antonin, W.; Fernandez-Chacon, R.; de Toledo, G. A.; Jo, T.; Geppert, M.; Valentijn, J. A.; Valentijn, K.; Jamieson, J. D.; Suedhof, T. C.; Jahn, R.
Date of Publication (YYYY-MM-DD):2002-09
Title of Journal:Molecular and Cellular Biology
Issue / Number:18
Start Page:6487
End Page:6497
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D- deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.
Comment of the Author/Creator:Date: 2002, SEP
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Reinhard Jahn / 190
MPI für biophysikalische Chemie/AG Dietmar Riedel/Elektronenmikroskopie
External Affiliations:Univ Seville, Dept Med Phys & Biophys, Sch Med, E-41009 Seville, Spain; Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA; Univ Utrecht, Fac Vet Med, Dept Biochem Cell Biol & Histol, NL-3584 CL Utrecht, Netherlands; Univ Texas, Ctr Basic Neurosci, Dept Mol Genet, Dallas, TX 75390 USA; Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75390 USA
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