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          Institute: MPI für biophysikalische Chemie     Collection: Spektroskopie und photochemische Kinetik (Prof. Jürgen Troe)     Display Documents

ID: 16665.0, MPI für biophysikalische Chemie / Spektroskopie und photochemische Kinetik (Prof. Jürgen Troe)
Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes
Authors:Swamy, M. J.; Marsh, D.; Anbazhagan, V.; Ramakrishnan, M.
Date of Publication (YYYY-MM-DD):2002-09-25
Title of Journal:FEBS Letters
Issue / Number:1-3
Start Page:230
End Page:234
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215-2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Free Keywords:BSP-Al; lipid-protein interaction; lipid bilayer; cholesterol efflux; phosphatidylcholine; sphingomyelin
Comment of the Author/Creator:Date: 2002, SEP 25
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Jürgen Troe / 010
External Affiliations:Univ Hyderabad, Sch Chem, Hyderabad 500046, Andhra Pradesh,; India;
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