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          Institute: MPI für biophysikalische Chemie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



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ID: 16705.0, MPI für biophysikalische Chemie / Abteilungsunabhängige Arbeitsgruppen
Production of maternal-zygotic mutant zebrafish by germ-line replacement
Authors:Ciruna, B.; Weidinger, G.; Knaut, H.; Thisse, B.; Thisse, C.; Raz, E.; Schier, A. F.
Language:English
Date of Publication (YYYY-MM-DD):2002-11-12
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Volume:99
Issue / Number:23
Start Page:14919
End Page:14924
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. Successful transfers are identified by the localization of specifically labeled donor PGCs to the region of the developing gonad in chimeric embryos. This strategy, which results in the complete replacement of the host germ line with donor PGCs, was validated by the generation of maternal and maternal-zygotic mutants for the miles apart locus. This germ-line replacement technique provides a powerful tool for studying the maternal effects of zygotic lethal mutations. Furthermore, the ability to generate large clutches of purely mutant embryos will greatly facilitate embryological, genetic, genomic, and biochemical studies.
Comment of the Author/Creator:Date: 2002, NOV 12
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/AG Erez Raz
External Affiliations:NYU, Sch Med, Skirball Inst Biomol Med, Dev Genet Program,Lab; 14, 540 1st Ave,4th Floor, New York, NY 10016 USA; NYU, Sch Med, Skirball Inst Biomol Med, Dev Genet Program,Lab 14, New York, NY 10016 USA; NYU, Sch Med, Dept Cell Biol, New York, NY 10016 USA; Max Planck Inst Dev Biol, Div 3, D-72076 Tübingen, Germany; Univ Strasbourg 1, Ctr Univ Strasbourg, INSERM, Inst Genet & Biol Mol & Cellulaire,CNRS, F-67404 Illkirch Graffenstaden, France
Identifiers:URL:http://www.pnas.org/content/99/23/14919.full.pdf+h...
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