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          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Biologie (Dr. Thomas M. Jovin)     Display Documents

ID: 168886.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Algorithms and practical fluorescence models of the photosynthetic apparatus of red cyanobacteria and Cryptophyta designed for the fluorescence detection of red cyanobacteria and cryptophytes
Authors:Beutler, M.; Wiltshire, K. H.; Reineke, C.; Hansen, U. P.
Date of Publication (YYYY-MM-DD):2004-04-28
Title of Journal:Aquatic Microbial Ecology
Start Page:115
End Page:129
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In fluorometric phytoplankton analysis, the detection of red cyanobacteria is hampered by acclimation processes of the cyanobacterial photosynthetic apparatus and spectral interferences with Cryptophyta. In order to overcome these problems, a simplified energy distribution model accounting for energy pathways in the red cyanobacterial photosynthetic apparatus and the apparatus of Cryptophyta was developed. Mathematical equations were derived that enabled calculation of the pigment content of cryptophyta and red cyanobacteria in the same sample. Phytoplankton samples were excited with 7 excitation wavelengths and measured at 4 detection wavelengths (600, 620, 650 and 685 nm) in vivo. A non-linear fit procedure accounted for variations in the fluorescence excitation spectra of red cyanobacteria and Cryptophyta in the presence of other phytoplankton fluorescence signals. Comparison with chemical pigment estimations verified that the fluorometric pigment estimation yielded reasonable results, even in the presence of energy-state transitions.
Free Keywords:Phycobilisome · Energy distribution model · Phycoerythrin · Phycocyanin · Cyanobacteria · Fluorescence · Cryptophyta
Last Change of the Resource (YYYY-MM-DD):2004-07-01
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Max-Planck-Institut (MPI) für Limnologie, August-Thienemann-Straße 2, 24302 Plön, Germany; Biologische Anstalt Helgoland, In der Stiftung Alfred-Wegner-Institut, Postfach 180, 27483 Helgoland, Germany; Zentrum für Biochemie und Molekularbiologie (ZBM), Universität Kiel, Leibnizstr. 11, 24098 Kiel, Germany
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